Ali Asghar Ghaderi; Baratali Fakheri; Nafise Mahdinezhad; Saeede Saeedi
Abstract
Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus ...
Read More
Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus pyogenes ATCC 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274, Staphylococcus aureus ATCC 25923.The minimum inhibitory concentration (MIC) of thymus ethanol extract on the bacteria was determined. The linear sensitivity of the bacteria against some antibiotics was also measured by the standard method of Kirby-Bauer disk diffusion. Results The results showed that the thymus leaf alcoholic extract inhibited the bacterial growth in the evaluated concentrations. The MIC of 6/25 ppm was observed effective against S. pneumoniae, H. alvei, P. mirabilis, and S. marcescens.Conclusion The results of the current study indicated the high effect of thymus alcoholic extract on the pathogenic bacteria. However, the medicinal application of plants requires further investigations.
Mohammad Bokaeian; Faramarz Dahmardeh; Mohammad Sohrabi; Saeide Saeidi
Volume 22, Issue 5 , November and December 2015, , Pages 780-787
Abstract
Background: Infectious diseases are an important cause of mortality in the world. Because of resistance of some human pathogenic bacteria to antibiotics, use of natural and herbal medicine is increasing to control these factors. The aim of this study is to investigate the antibacterial effects of Ramalina ...
Read More
Background: Infectious diseases are an important cause of mortality in the world. Because of resistance of some human pathogenic bacteria to antibiotics, use of natural and herbal medicine is increasing to control these factors. The aim of this study is to investigate the antibacterial effects of Ramalina hyrcana Sipman lichens against many Gram-positive and Gram-negative bacteria.
Materials and Methods: The lichen ethanol extract was prepared by using a rotary machine. Nine standard strains of Gram- positive and Gram -negative bacteria were cultured in nutrient broth. The minimum inhibitory concentration (MIC) was determined by the micro dilution method.
Results: The results of this study showed that the lichen R. hyrcana extract in different concentrations inhibited both Gram-positive and Gram-negative bacteria. The results showed that the lichen extract of all concentrations inhibited of Staphylococcus aureus. After S. aureus bacteria inhibition, the minimum inhibitory concentrations are for Hafnia and Acinetobacter (12.5 ppm).
Conclusion: According to the results and other reports in this area, lichen R. hyrcana extract could be introduced as a biological control agent. However, for the use of different lichen extracts for clinical application, the chemical analysis of extracts and clinical research is necessary.
Seyyed Kazem Sabbagh; Saeedeh Saeedi; Zahra Dehbashi; Mahta Mazaheri Naeieni
Volume 22, Issue 5 , November and December 2015, , Pages 854-861
Abstract
Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative ...
Read More
Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative medicaments. The purpose of this study was to investigate the antimicrobial activity of alcoholic extract of Black pepper and March against clinical isolates of Staphylococcus aureus resistant to commonly used antibiotics.
Materials and Methods: Alcholic plant extraction was done using a rotary apparatus. Twelve, Staphylococcus aureus strains from urine samples was isolated and then purified. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBI) of the plant extracts at six different concentrations was determined broth medium using dilution method. Susceptibility of strains to multiple antibiotics was evaluated using Kirby-Bauer disk diffusion method.
Result: The results of this study showed that higher and lower average resistance to tested antibiotic is belonged to Ceftazidime (66/66%) and vancomycin (8/1%) antibiotic, respectively. MIC assay of plant extracts showed that the highest effect of Pepper and March was determined at concentrations of 10 and 5 mg/ml, respectively. Whereas, the lowest MIC effect for Pepper and March was recorded at concentration of 2/5 and 0/62 mg/mL, respectively. The maximum MBC of plant extracts was recorded at concentration of 20 and 10 mg/mL for Pepper and March, respectively.
Conclusion: Although the clinical application of plant extracts and essential oils due to fewer side effects, seems valuable, However, to clinical application of the alcoholic extract of black pepper and march more researches on mode of action of effective compounds of plans on microbial agents have to be done.